1UM8
Crystal structure of helicobacter pylori ClpX
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-27 |
Wavelength(s) | 0.9796, 0.9798, 0.9754 |
Spacegroup name | P 65 |
Unit cell lengths | 78.515, 78.515, 131.514 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
Rwork | 0.219 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.185 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.059 | 0.326 |
Number of reflections | 13273 | 1369 * |
<I/σ(I)> | 22.5 | 2.6 |
Completeness [%] | 93.7 | 96.3 |
Redundancy | 3.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Kim, D.Y., (2003) Acta Cryst., D59, 1642. * |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Kim, D.Y., (2003) Acta Cryst., D59, 1642. * |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Kim, D.Y., (2003) Acta Cryst., D59, 1642. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | imidazole | 100 (mM) | pH8.0 |
3 | 1 | reservoir | ammonium phosphate | 1.0 (M) | |
4 | 1 | reservoir | 0.2 (M) |