1ULV
Crystal Structure of Glucodextranase Complexed with Acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.795, 88.247, 80.994 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 49.600 * - 2.420 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 * | 2.510 |
| High resolution limit [Å] | 2.420 * | 2.420 |
| Rmerge | 0.069 * | 0.206 * |
| Total number of observations | 184944 * | |
| Number of reflections | 48692 | |
| Completeness [%] | 98.8 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 20 * | PEG 8000, potassium dihydrogen phosphate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 20 (mM) | pH6.0 |
| 3 | 1 | drop | calcium acetate | 5 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 3.0 (%(w/v)) | |
| 5 | 1 | reservoir | potassium dihydrogen phosphate | 80 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.1 |
