1ULT

Crystal structure of tt0168 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-10
Detector	MARRESEARCH
Wavelength(s)	0.7090
Spacegroup name	P 21 21 21
Unit cell lengths	55.952, 124.687, 212.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.640 - 2.550
R-factor	0.206
Rwork	0.206
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	48374
Completeness [%]	97.3	90.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG4000, ammonium sulfate, Na citrate, guanidine hydrochloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K