1ULS

Crystal structure of tt0140 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-07
Detector	RIGAKU RAXIS V
Wavelength(s)	1.020
Spacegroup name	P 1
Unit cell lengths	77.260, 77.330, 78.670
Unit cell angles	90.12, 90.96, 95.12

Refinement procedure

Resolution	46.400 - 2.400
R-factor	0.196
Rwork	0.196
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	69802
Completeness [%]	98.2	95.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP