1ULK
Crystal Structure of Pokeweed Lectin-C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Detector technology | CCD |
| Collection date | 2001-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.122, 104.122, 69.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.500 * - 1.800 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.20300 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.500 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.279 |
| Total number of observations | 134896 * | |
| Number of reflections | 26091 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 25 * | sodium acetate, ammonium sulfate, dioxane, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 0.1 (M) | pH7.5 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
