1ULH
A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9799 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.025, 96.606, 98.319 |
| Unit cell angles | 90.00, 130.26, 90.00 |
Refinement procedure
| Resolution | 42.890 - 2.310 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| RMSD bond length | 0.073 * |
| RMSD bond angle | 1.410 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.056 * | 0.215 * |
| Number of reflections | 106623 | |
| Completeness [%] | 90.1 | 76.4 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | PEG 10000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | Bis-Tris | 100 (mM) | pH5.5 |
| 6 | 1 | reservoir | ammonium acetate | 80-100 (mM) | |
| 7 | 1 | reservoir | PEG10000 | 8-12 (%(w/v)) |
