1ULG
CGL2 in complex with Thomsen-Friedenreich antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.137, 113.634, 65.091 |
Unit cell angles | 90.00, 108.48, 90.00 |
Refinement procedure
Resolution | 29.790 - 2.200 |
Rwork | 0.219 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CGL2-lactose |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.790 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 20221 | |
<I/σ(I)> | 12.9 | 6.1 |
Completeness [%] | 67.4 | 67.4 |
Redundancy | 1.8 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | PEG 3350, PEG 400, sodium phosphate, sodium chloride, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |