1UKY

SUBSTRATE SPECIFICITY AND ASSEMBLY OF CATALYTIC CENTER DERIVED FROM TWO STRUCTURES OF LIGATED URIDYLATE KINASE

Experimental procedure

Spacegroup name	P 61 2 2
Unit cell lengths	64.200, 64.200, 185.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.130
R-factor	0.178
Rwork	0.178
RMSD bond length	0.018
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.130 *	2.130 *
Total number of observations	32483 *
Number of reflections	12091 *
Completeness [%]	91.0 *	64 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	uridylate kinase	5 (mg/ml)
2	1	drop	PEG3350	10 (%(w/v))
3	1	drop	n-octyl-beta-D-glucoside	0.02 (%)
4	1	drop	ATP	1.5-2.0 (mM)	or ADP
5	1	drop	potassium phosphate	50 (mM)
6	1	reservoir	PEG3350	15 (%(w/v))
7	1	reservoir	potassium phosphate	50 (mM)