1UKV
Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9340 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.503, 119.577, 60.723 |
Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
Resolution | 19.490 - 1.500 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 106322 | |
<I/σ(I)> | 11.65 | 3.15 |
Completeness [%] | 98.4 | 96.3 |
Redundancy | 3.6 | 3.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 16% PEG 8000, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |