1UKU
Crystal Structure of Pyrococcus horikoshii CutA1 Complexed with Cu2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 52.242, 52.242, 54.111 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.450 |
| R-factor | 0.16488 |
| Rwork | 0.162 |
| R-free | 0.19415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j2v |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.478 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 15562 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 100mM acetate buffer, 1.5M ammonium sulfate, 2mM CuSO4, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
