1UKT
Crystal structure of Y100L mutant cyclodextrin glucanotransferase compexed with an acarbose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02-22 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 65.680, 74.470, 80.100 |
Unit cell angles | 85.29, 105.59, 100.81 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.179 |
Rwork | 0.173 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pam |
RMSD bond length | 0.009 |
RMSD bond angle | 1.520 |
Data reduction software | FFFEAR |
Data scaling software | FFFEAR |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.770 | 2.050 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.068 | 0.371 |
Number of reflections | 68926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 5.6 | 293 | Harata, K., (1996) Acta Cryst., D52, 1136. * |