1UKQ
Crystal structure of cyclodextrin glucanotransferase complexed with a pseudo-maltotetraose derived from acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2001-10-22 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.940, 74.490, 79.100 |
| Unit cell angles | 85.13, 105.02, 101.02 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pam |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 3.010 * |
| Data reduction software | FFFEAR |
| Data scaling software | FFFEAR |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 * | 2.039 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.132 | 0.245 |
| Number of reflections | 91157 | |
| Completeness [%] | 96.0 | 86.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 293 | Harata, K., (1996) Acta Cryst., D52, 1136. * |
