1UKP
Crystal structure of soybean beta-amylase mutant substituted at surface region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-17 |
Detector | OXFORD PX210 |
Wavelength(s) | 0.900 |
Spacegroup name | P 1 |
Unit cell lengths | 75.463, 78.801, 88.560 |
Unit cell angles | 89.92, 90.21, 89.90 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23450 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1byb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.273 * |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.980 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | 0.383 |
Total number of observations | 335804 * | |
Number of reflections | 137751 | 13776 * |
Completeness [%] | 95.4 | 95.3 |
Redundancy | 2.44 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 4 * | ammonium sulfate, sodium acetate, 2-mercaptoethanol, EDTA, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 45-50 (%) | |
3 | 1 | reservoir | EDTA | 1 (mM) | |
4 | 1 | reservoir | 2-ME | 18 (mM) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.4 |