1UKK
Structure of Osmotically Inducible Protein C from Thermus thermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97894, 0.97910, 0.97920, 0.97500, 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.580, 40.950, 48.140 |
| Unit cell angles | 76.93, 74.04, 64.05 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.18068 |
| Rwork | 0.178 |
| R-free | 0.23222 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.899 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.033 | 0.162 |
| Number of reflections | 30286 | |
| <I/σ(I)> | 15 | 4.1 |
| Completeness [%] | 90.4 | 79.3 |
| Redundancy | 1.85 | 1.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 296 | PEG 4000, Tris-HCl, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
