1UKF

Crystal Structure of Pseudomonas Avirulence Protein AvrPphB

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-04
Detector	CUSTOM-MADE
Wavelength(s)	0.9792, 0.9879, 0.9793, 0.9565
Spacegroup name	P 21 21 21
Unit cell lengths	43.601, 49.596, 75.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.090 - 1.350
R-factor	0.204
Rwork	0.204
R-free	0.22000 *
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	22.100 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.090	1.350
High resolution limit [Å]	1.350 *	1.300
Rmerge	0.036 *	0.232 *
Total number of observations	236773 *
Number of reflections	39746
<I/σ(I)>		2
Completeness [%]	96.2	93.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	293	Ammonium Sulfate, PIPES, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.5
2	1	drop		10 (mM)
3	1	drop	TCEP-HCl	1 (mM)
4	1	drop	protein	17 (mg/ml)
5	1	reservoir	ammonium sulfate	1.95 (M)
6	1	reservoir	PIPES	100 (mM)	pH6.7