1UJ8

Structures of ORF3 in Two Crystal Forms, a Member of Isc Machinery of E. coli Involved in the Assembly of Iron-Sulfur Clusters

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-19
Detector	MARRESEARCH
Wavelength(s)	0.9740, 0.9801
Spacegroup name	C 1 2 1
Unit cell lengths	76.630, 27.250, 39.680
Unit cell angles	90.00, 111.00, 90.00

Refinement procedure

Resolution	19.840 - 1.750
Rwork	0.194
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.055	0.224
Number of reflections	7922
Completeness [%]	99.9	100
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	sodium citrate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	sodium citrate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K