1UJ5
Crystal structure of Thermus thermophilus ribose-5-phosphate isomerase complexed with ribose-5-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-10 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.892, 62.009, 131.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.810 - 2.000 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 17862 | |
Completeness [%] | 97.7 | 82.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 8.4 | 291 | PEG4000, Tris, LiCl, ribose-5-phosphate, pH 8.4, LIQUID DIFFUSION, temperature 291K |