1UJ4
Crystal structure of Thermus thermophilus ribose-5-phosphate isomerase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.900, 0.9791, 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.101, 61.965, 131.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.860 - 1.800 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 23909 | |
| Completeness [%] | 99.8 | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8.4 | 291 | PEG4000, Tris, LiCl, pH 8.4, LIQUID DIFFUSION, temperature 291K |
