1UJ4
Crystal structure of Thermus thermophilus ribose-5-phosphate isomerase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-15 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.900, 0.9791, 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.101, 61.965, 131.342 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.860 - 1.800 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.21500 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 23909 | |
Completeness [%] | 99.8 | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 8.4 | 291 | PEG4000, Tris, LiCl, pH 8.4, LIQUID DIFFUSION, temperature 291K |