1UIS

The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-06-20
Detector	RIGAKU RAXIS IV
Spacegroup name	P 65 2 2
Unit cell lengths	77.935, 77.935, 327.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.000
R-factor	0.2526
Rwork	0.213
R-free	0.25300 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.370 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.046 *	0.230 *
Total number of observations	134722 *
Number of reflections	37625 *
Completeness [%]	91.2 *	79.7
Redundancy	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.3	20 *	PEG 8000, sodium acetate, calcium acetate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	26 (mg/ml)
2	1	reservoir	PEG8000	11.5 (%)
3	1	reservoir	sodium acetate	0.1 (%)	pH4.3
4	1	reservoir	calcium acetate	0.2 (M)