1UII
Crystal structure of Geminin coiled-coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-04 |
Detector | BRANDEIS |
Wavelength(s) | 0.9782, 0.9783, 0.9789, 0.9611 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.160, 94.460, 102.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.23900 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO (1.97) |
Data scaling software | SCALEPACK (1.97) |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 25309 | |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 6.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 293 | 0.36M Sodium Acetate, 0.1M TRIS, 30% PEG 4000, pH 8.5, EVAPORATION, temperature 293K |
1 | EVAPORATION | 8.5 | 293 | 0.36M Sodium Acetate, 0.1M TRIS, 30% PEG 4000, pH 8.5, EVAPORATION, temperature 293K |