1UIA

ANALYSIS OF THE STABILIZATION OF HEN LYSOZYME WITH THE HELIX DIPOLE AND CHARGED SIDE CHAINS

Experimental procedure

Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1995-08-30
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.720, 79.720, 37.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.760
R-factor	0.18
Rwork	0.180
Starting model (for MR)	1rfp
RMSD bond length	0.009
RMSD bond angle	23.987 *
Data reduction software	PROCESS ((SCALE))
Data scaling software	PROCESS ((SCALE))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.810
High resolution limit [Å]	1.760	1.757
Rmerge	0.048	0.284
Number of reflections	10820
Completeness [%]	86.2	59.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.7	23 *	50 MM ACETATE AT PH 4.7 CONTAINING 0.9 M NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.9 (M)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	drop	protein	20 (mg/ml)
4	1	drop	sodium acetate	0.1 (M)