1UI9
Crystal analysis of chorismate mutase from thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-06-23 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 3 |
Unit cell lengths | 74.804, 74.804, 74.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.450 - 1.650 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ufy |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.046 | 0.328 |
Number of reflections | 16749 | |
Completeness [%] | 97.9 | 99.9 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.3 | 295 | Sodium malonate, MES, pH 6.3, Micro Batch, temperature 295K |