1UI8
Site-directed mutagenesis of His592 involved in binding of copper ion in Arthrobacter globiformis amine oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.85 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.762, 63.210, 184.244 |
| Unit cell angles | 90.00, 111.64, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.218 |
| Rwork | 0.217 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | |
| Number of reflections | 220460 | |
| Completeness [%] | 99.3 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | potassium sodium tartrate, pH 6.8, MICRODIALYSIS, temperature 289K |
