1UI0
Crystal Structure Of Uracil-DNA Glycosylase From Thermus Thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-23 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.429, 59.512, 80.971 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Selenomethionyl uracil-DNA glycosylase from Thermus thermophilus HB8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.180 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.079 * | 0.133 * |
| Total number of observations | 163400 * | |
| Number of reflections | 35830 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | 1.2-1.5M ammonium sulfate, 75mM Tris, 25% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.3 (M) | |
| 3 | 1 | drop | glycerol | 25 (%) | |
| 4 | 1 | drop | Tris-HCl | 75 (mM) | pH8.5 |
