1UHH
Crystal structure of cp-aequorin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD PX210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.198, 57.274, 58.191 |
| Unit cell angles | 72.20, 80.00, 68.40 |
Refinement procedure
| Resolution | 33.480 - 1.800 |
| R-factor | 0.14458 |
| Rwork | 0.142 |
| R-free | 0.18954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ej3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.810 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 36134 | |
| <I/σ(I)> | 9.3 | 4.5 |
| Completeness [%] | 95.2 | 95.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammonium sulfate, pH 7.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
