1UHA
Crystal Structure of Pokeweed Lectin-D2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 2000-03-02 |
Detector | WEISSENBERG |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 23.240, 56.950, 29.620 |
Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
Resolution | 27.960 - 1.500 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 11513 | |
Completeness [%] | 98.5 | 95.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 4.6 | 298 | PEG 8000, 0.2M calcium acetate, 0.1M sodium acetate buffer, pH 4.6, SMALL TUBES, temperature 298K |