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1UGY

Crystal structure of jacalin- mellibiose (Gal-alpha(1-6)-Glc) complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]293
Detector technologyIMAGE PLATE
DetectorMARRESEARCH
Wavelength(s)1.5418
Spacegroup nameP 21 21 21
Unit cell lengths80.460, 99.830, 105.650
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000

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- 2.400
R-factor0.18
Rwork0.180
R-free0.21400
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006
RMSD bond angle26.500

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.490
High resolution limit [Å]2.4002.400
Rmerge0.1380.400
Total number of observations106303

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Number of reflections323313044

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Completeness [%]95.291.1
Redundancy3.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

7.3293PEG 4000, ammonium sulfate, sodium acetate trihydrate buffer, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropphosphate0.02 (M)pH7.3
31drop0.1 (M)
41dropsodium azide0.025 (%(w/v))
51dropPEG40008-10 (%)
61reservoirPEG400040 (%)

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PDB entries from 2024-03-27

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