1UG9
Crystal Structure of Glucodextranase from Arthrobacter globiformis I42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 199.860, 88.535, 80.781 |
Unit cell angles | 90.00, 113.08, 90.00 |
Refinement procedure
Resolution | 19.900 * - 2.420* |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.22700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lf6 |
RMSD bond length | 0.006 |
RMSD bond angle | 24.900 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.590 |
High resolution limit [Å] | 2.420 * | 2.420 * |
Rmerge | 0.070 * | 0.223 * |
Total number of observations | 193427 * | |
Number of reflections | 48376 * | |
Completeness [%] | 96.0 * | 62.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 20 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | sodium acetate | 20 (mM) | pH6.0 |
3 | 1 | drop | calcium acetate | 5 (mM) | |
4 | 1 | reservoir | PEG8000 | 3.0 (%(w/v)) | |
5 | 1 | reservoir | potassium dihydrogen phosphate | 80 (mM) | |
6 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.1 |