1UG3
C-terminal portion of human eIF4GI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-02-19 |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.618, 50.567, 99.895 |
| Unit cell angles | 90.00, 101.59, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.240 |
| R-factor | 0.247 |
| Rwork | 0.244 |
| R-free | 0.29200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.310 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 36093 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 96.4 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG MME 5000, 0.1M MES, 0.2M AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
