1UFQ

Crystal structure of ligand-free human uridine-cytidine kinase 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-27
Detector	OXFORD
Wavelength(s)	0.9000
Spacegroup name	P 21 21 2
Unit cell lengths	84.057, 94.262, 156.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.410 - 2.500
R-factor	0.237
Rwork	0.236
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1udw
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.410	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.052	0.217
Number of reflections	38404
<I/σ(I)>	12	3.2
Completeness [%]	87.4	90.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	PEG400, PEG3350, glycerol, HEPES, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K