1UFH
Structure of putative acetyltransferase, YYCN protein of Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792, 0.9565 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.260, 88.580, 55.800 |
| Unit cell angles | 90.00, 98.80, 90.00 |
Refinement procedure
| Resolution | 26.080 - 2.200 |
| R-factor | 0.2339 |
| Rwork | 0.233 |
| R-free | 0.25900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.260 * |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.080 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 * | 0.238 * |
| Number of reflections | 21192 * | |
| <I/σ(I)> | 6.8 | 18.8 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 22 * | 900mM Ammonium phosphate, 190mM Sodium chloride, 100mM Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium phosphate | 900 (mM) | |
| 3 | 1 | reservoir | 190 (mM) | ||
| 4 | 1 | reservoir | imidazole | 100 (mM) | pH8.0 |
