1UFA
Crystal structure of TT1467 from Thermus thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 119.376, 119.376, 73.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.200 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.23800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 29307 | |
Completeness [%] | 98.7 | 89.1 |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Lithium Sulfate, Sodium Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |