1UF2
The Atomic Structure of Rice dwarf Virus (RDV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 285 |
Detector technology | DIFFRACTOMETER |
Collection date | 1998-10-14 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 770.000, 795.000, 814.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 228.740 - 3.500 |
R-factor | 0.303 |
Rwork | 0.303 |
R-free | 0.30600 |
Structure solution method | Molecular Replacement Averaging |
Starting model (for MR) | Cryo-electronmicrograph |
RMSD bond length | 0.010 |
RMSD bond angle | 1.480 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | RAVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 230.000 | 3.590 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.186 | 0.783 |
Total number of observations | 17806888 * | |
Number of reflections | 3001937 * | |
<I/σ(I)> | 3.1 | 0.9 |
Completeness [%] | 97.7 | 91.5 |
Redundancy | 5.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Mizuno, H., (1991) J. Mol. Biol., 219, 665. * |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Mizuno, H., (1991) J. Mol. Biol., 219, 665. * |