1UEU
Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 0.9704 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.835, 78.111, 77.815 |
Unit cell angles | 90.00, 97.65, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.000 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | |
High resolution limit [Å] | 2.000 | |
Rmerge | 0.063 * | 0.356 * |
Number of reflections | 31484 | |
Completeness [%] | 90.5 * | 80.3 * |
Redundancy | 2.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 20 * | PEG4000, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3 (mg/ml) | |
10 | 1 | reservoir | 1,6-hexanediol | 5.6 (M) | |
11 | 1 | reservoir | 0.4 (M) | ||
2 | 1 | drop | Tris-HCl | 45 (mM) | pH7.8 |
3 | 1 | drop | PEG4000 | 10.8 (%) | |
4 | 1 | drop | calcium acetate | 135 (mM) | |
5 | 1 | drop | 1,6-hexanediol | 6.3 (%) | |
6 | 1 | drop | dithiothreitol | 10 (mM) | |
7 | 1 | reservoir | Tris-HCl | 40 (mM) | pH7.8 |
8 | 1 | reservoir | PEG4000 | 9.6 (%) | |
9 | 1 | reservoir | calcium acetate | 120 (mM) |