1UEJ
Crystal structure of human uridine-cytidine kinase 2 complexed with a substrate, cytidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2002-10-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9795 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 138.296, 247.631, 90.944 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.990 - 2.610 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1udw |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.084 | 0.270 |
Number of reflections | 23533 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 98.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | sodium citrate, HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |