1UEG
Crystal structure of amino-terminal microtubule binding domain of EB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 48.589, 48.589, 90.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.880 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27900 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.109 | 0.282 |
| Number of reflections | 4615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | ammonium sulfate, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
