1UEC
Crystal structure of autoinhibited form of tandem SH3 domain of p47phox
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-09 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.217, 100.217, 44.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.990 - 1.820 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.050 | 0.274 |
| Total number of observations | 124911 * | |
| Number of reflections | 21103 | |
| <I/σ(I)> | 14.6 | 3.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | Yuzawa, S., (2003) Acta Crystallogr., D59, 1479. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-35 (mg/ml) | |
| 2 | 1 | drop | sodium fluoride | 50 (mM) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.4-5.6 |
| 4 | 1 | reservoir | PEG6000 | 16-18 (%) |
