1UE0
Isoleucyl-tRNA synthetase editing domain complexed with L-Valine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.270, 102.270, 84.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.940 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.24800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IleRS CP1 domain |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.650 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.047 | 0.285 * |
| Number of reflections | 30184 | |
| <I/σ(I)> | 7.1 | 2.6 |
| Completeness [%] | 98.8 | 99.2 |
| Redundancy | 8.9 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | ammonium sulfate,2-propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | HEPES-NaOH | 50 (mM) | pH7.0 |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | reservoir | 2-propanol | 5 (%) | |
| 5 | 1 | reservoir | valine | 100 (mM) |
