1UDZ
Isoleucyl-tRNA synthetase editing domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 102.739, 102.739, 83.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.950 - 1.800 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CP1 domain of the full-length T. thermophilus IleRS |
RMSD bond length | 0.020 * |
RMSD bond angle | 1.980 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.282 |
Number of reflections | 41489 * | |
<I/σ(I)> | 45.3 | 3.8 |
Completeness [%] | 99.1 * | 98.2 |
Redundancy | 15.8 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | ammonium sulfate,2-propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | HEPES-NaOH | 50 (mM) | pH7.0 |
3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
4 | 1 | reservoir | 2-propanol | 5 (%) |