1UDW
Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | OXFORD |
| Wavelength(s) | 0.9000 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 133.204, 248.097, 91.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.470 - 2.600 |
| R-factor | 0.214 |
| Rwork | 0.210 |
| R-free | 0.25100 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.283 |
| Number of reflections | 23389 | |
| <I/σ(I)> | 10.5 | 2.4 |
| Completeness [%] | 99.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | sodium tartrate, HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
