1UDR
CHEY MUTANT WITH LYS 91 REPLACED BY ASP, LYS 92 REPLACED BY ALA, ILE 96 REPLACED BY LYS AND ALA 98 REPLACED BY LEU (STABILIZING MUTATIONS IN HELIX 4)
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.850, 109.760, 54.000 |
Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.22200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3chy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.280 |
Data reduction software | XDS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.900 * | 1.900 * |
Rmerge | 0.077 * | |
Number of reflections | 42474 | |
Completeness [%] | 87.2 * | 82.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.9 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 1.2 (M) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 2.4 (M) |