1UDC

STRUCTURE OF UDP-GALACTOSE-4-EPIMERASE COMPLEXED WITH UDP-MANNOSE

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	83.500, 83.500, 108.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.177 *
Rwork	0.177
RMSD bond length	0.013
RMSD bond angle	14.700 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.730
High resolution limit [Å]	1.650 *	1.650
Rmerge	0.033 *	0.182
Total number of observations	160336 *
Number of reflections	50521	5849 *
Completeness [%]	95.0	89
Redundancy	3.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20-25 (%)
2	1	reservoir		250-500 (mM)
3	1	reservoir	CHES	50 (mM)