1UD0
CRYSTAL STRUCTURE OF THE C-TERMINAL 10-kDA SUBDOMAIN OF HSC70
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.9793, 0.940, 0.9802 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 117.479, 117.479, 163.777 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.930 - 3.450 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.30900 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.580 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.084 | 0.196 |
| Number of reflections | 62784 | |
| <I/σ(I)> | 6.4 | 5.4 |
| Completeness [%] | 98.7 | 98.8 |
| Redundancy | 6.9 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 298 | ammonium sulfate, 2-propanol, sodium acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
