1UCW

COMPLEX OF TRANSALDOLASE WITH THE REDUCED SCHIFF-BASE INTERMEDIATE

Experimental procedure

Source type	SEALED TUBE
Detector technology	IMAGE PLATE
Collection date	1996-03-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	69.200, 91.900, 130.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.204
Rwork	0.204
R-free	0.24400
RMSD bond length	0.008
RMSD bond angle	1.481
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.250
High resolution limit [Å]	2.200 *	2.200
Rmerge	0.099
Total number of observations	173783 *
Number of reflections	37868
Completeness [%]	87.0	77.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *	293 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.1 (M)
2	1	reservoir	PEG6000	15 (%)