1UCS
Type III Antifreeze Protein RD1 from an Antarctic Eel Pout
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-01-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.6668 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.501, 39.502, 44.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 0.620 |
| R-factor | 0.137 |
| Rwork | 0.133 |
| R-free | 0.15500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A polypeptide model derived from NMR experiments |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.200 * | 0.640 |
| High resolution limit [Å] | 0.620 | 0.620 |
| Rmerge | 0.073 * | 0.653 |
| Total number of observations | 256420 * | |
| Number of reflections | 108526 * | 10198 * |
| <I/σ(I)> | 21.5 | 1.2 |
| Completeness [%] | 97.0 * | 91.8 |
| Redundancy | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium Sulfate, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | Tris | 4 (mM) | pH7.5 |
| 3 | 1 | drop | ammonium sulfate | 20 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 50 (%) |
