1UCL

Mutants of RNase Sa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	IMAGE PLATE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.261, 77.658, 38.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.820
R-factor	0.176
Rwork	0.176
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.820
Rmerge	0.062 *
Total number of observations	98889 *
Number of reflections	17544 *
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	2.0M ammonium sulfate, 0.1M HEPES-Na pH 7.5, 2%(v/v) PEG400, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	0.1 (M)	pH8.4
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	ammonium sulfate	2.0 (M)
4	1	reservoir	sodium HEPES	0.1 (M)	pH7.5
5	1	reservoir	PEG400	2 (%(v/v))