1UCJ
Mutants of RNase Sa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.340, 77.819, 38.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.800* |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 24.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 |
Rmerge | 0.055 * |
Total number of observations | 75244 * |
Number of reflections | 17987 |
Completeness [%] | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.6M ammonium sulfate, 0.1M MES pH 6.5, 10%(v/v) dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium phosphate | 0.2 (M) | pH7.0 |
2 | 1 | drop | protein | 20 (mg/ml) | |
3 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
4 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
5 | 1 | reservoir | dioxane | 10 (%) |