1UCJ

Mutants of RNase Sa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	120
Detector technology	IMAGE PLATE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	64.340, 77.819, 38.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.800 *
R-factor	0.185
Rwork	0.185
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800
Rmerge	0.055 *
Total number of observations	75244 *
Number of reflections	17987
Completeness [%]	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.6M ammonium sulfate, 0.1M MES pH 6.5, 10%(v/v) dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	0.2 (M)	pH7.0
2	1	drop	protein	20 (mg/ml)
3	1	reservoir	ammonium sulfate	1.6 (M)
4	1	reservoir	MES	0.1 (M)	pH6.5
5	1	reservoir	dioxane	10 (%)