1UCD

Crystal structure of Ribonuclease MC1 from bitter gourd seeds complexed with 5'-UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-21
Detector	MARRESEARCH
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	39.180, 63.480, 73.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.300
Rwork	0.200
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bk7
RMSD bond length	0.005
RMSD bond angle	1.359
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	1.370
High resolution limit [Å]	1.300	1.300
Rmerge	0.052	0.239
Number of reflections	45999
<I/σ(I)>	8.7	2.4
Completeness [%]	99.9	99.4
Redundancy	7.1	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	PEG 8000, sodium acetate, sodium cacodylate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K