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1UCC

Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL44B2
Synchrotron siteSPring-8
BeamlineBL44B2
Temperature [K]298
Detector technologyCCD
Collection date1999-10-12
DetectorMARRESEARCH
Wavelength(s)0.7
Spacegroup nameP 21 21 21
Unit cell lengths38.800, 67.630, 75.540
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 1.770
Rwork0.181
R-free0.21400
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1bk7
RMSD bond length0.005
RMSD bond angle1.686

*

Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]19.400

*

1.870
High resolution limit [Å]1.7701.770
Rmerge0.0860.370

*

Total number of observations427770

*

Number of reflections18370
<I/σ(I)>6.62
Completeness [%]92.794.3
Redundancy4.34.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.7293PEG 8000, sodium acetate, sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein19 (mg/ml)
21reservoirsodium acetate0.2 (M)
31reservoirsodium cacodylate0.1 (M)pH6.7
41reservoirPEG800021 (%)
51reservoir3'-UMP10 (mM)

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