1UCC
Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 1999-10-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.7 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.800, 67.630, 75.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.770 |
Rwork | 0.181 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bk7 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.686 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 * | 1.870 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.086 | 0.370 * |
Total number of observations | 427770 * | |
Number of reflections | 18370 | |
<I/σ(I)> | 6.6 | 2 |
Completeness [%] | 92.7 | 94.3 |
Redundancy | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG 8000, sodium acetate, sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 19 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 0.2 (M) | |
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.7 |
4 | 1 | reservoir | PEG8000 | 21 (%) | |
5 | 1 | reservoir | 3'-UMP | 10 (mM) |