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1UC8

Crystal structure of a lysine biosynthesis enzyme, Lysx, from thermus thermophilus HB8

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL45PX
Synchrotron siteSPring-8
BeamlineBL45PX
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2002-06-04
DetectorRIGAKU RAXIS IV
Wavelength(s)1.02
Spacegroup nameC 1 2 1
Unit cell lengths124.652, 51.366, 103.594
Unit cell angles90.00, 122.81, 90.00
Refinement procedure
Resolution43.500

*

- 2.000
Rwork0.240
R-free0.26900

*

Structure solution methodMAD
RMSD bond length0.006
RMSD bond angle23.200

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Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSOLVE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.070
High resolution limit [Å]2.0002.000
Rmerge0.0530.475
Number of reflections37382
<I/σ(I)>12.82.9
Completeness [%]99.699.9
Redundancy4.24.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP4.6293Vassylyeva, M.N., (2003) Acta Crystallogr., D59, 1651.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21reservoirPEG40005 (%)
31reservoirsodium acetate17 (mM)pH4.6
41reservoirammonium acetate0.35 (mM)

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